Regulation of Periplasmic Carbonic Anhydrase Expression in Chlamydomonas reinhardtii by Acetate and pH
نویسندگان
چکیده
منابع مشابه
Periplasmic carbonic anhydrase structural gene (Cah1) mutant in chlamydomonas reinhardtii
To survive in various conditions of CO2 availability, Chlamydomonas reinhardtii shows adaptive changes, such as induction of a CO2-concentrating mechanism, changes in cell organization, and induction of several genes, including a periplasmic carbonic anhydrase (pCA1) encoded by Cah1. Among a collection of insertionally generated mutants, a mutant has been isolated that showed no pCA1 protein an...
متن کاملcDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration.
cDNA clones for the periplasmic carbonic anhydrase (CA; carbonate hydro-lyase, EC 4.2.1.1) of Chlamydomonas reinhardtii cells were isolated and characterized. The fact that the cloned cDNA fragments encoded a 377-amino acid polypeptide (41,626 Da) consisting of an NH2-terminal hydrophobic signal peptide of 20 amino acids, a large (35,603 Da) subunit polypeptide, and a small (4144 Da) subunit po...
متن کاملLight-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii.
The effect of external inorganic carbon concentration and light on carbonic anhydrase (CA) protein accumulation and steady-state mRNA levels were examined in Chlamydomonas reinhardtii. When photoautotrophically grown high-CO(2) cells were transferred to low-CO(2) conditions, they exhibited a significant accumulation of the 2.0-kilobase CA transcript after 1 hour with the maximum level reached a...
متن کاملIdentification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii.
Carbonic anhydrases (CA) are zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. The three evolutionarily unrelated families of CAs are designated alpha-, beta-, and gamma-CA. Aquatic photosynthetic organisms have evolved different forms of CO2 concentrating mechanisms (CCMs) to aid Rubisco in capturing CO2 from the surrounding environment. One aspect of all CCMs is th...
متن کاملIdentification of Extracellular Carbonic Anhydrase of Chlamydomonas reinhardtii.
We have examined the induction of carbonic anhydrase activity in Chlamydomonas reinhardtii and have identified the polypeptide responsible for this activity. This polypeptide was not synthesized when the alga was grown photoautotrophically on 5% CO(2), but its synthesis was induced under low concentrations of CO(2) (air levels of CO(2)). In CW-15, a mutant of C. reinhardtii which lacks a cell w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1994
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.106.1.103